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Characteristics of Microbes
• Prokaryote
• microscopic, unicellular organisms
• lack nuclei and membrane-bound organelles
Bacterial Structures
•   Flagella
•   Pili
•   Capsule
•   Plasma Membrane
•   Cytoplasm
•   Cell Wall
•   Lipopolysaccharides
•   Teichoic Acids
•   Inclusions
•   Spores
                                   Chapter 4
How to Make a Dichotomous
Key From a Table of Bacterial
       Characteristics

 (for use in running the minimum
 number of tests in identifying an
       unknown bacterium)
Table of Characteristics for Eight Bacteria
      Organism                                       Characteristics
Alcaligenes faecalis     Gram negative bacillus, acid-fast negative, ferments galactose,
                         catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S
                         negative
Citrobacter freundii     Gram negative bacillus; acid-fast negative, does not ferment galactose,
                         catalase positive, non-motile, acid produced in litmus milk, not
                         hemolytic, H2S negative

Pseudomonas              Gram negative bacillus, acid-fast negative, does not ferment galactose,
fluorescens              catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S
                         negative
Streptococcus            Gram positive oval coccus found as streptococci, acid-fast negative,
pneumoniae               galactose fermentation negative, catalase negative, non-motile, acid in
                         litmus milk, hemolytic, H2S negative

Mycobacterium            Gram positive club-shaped bacilli, acid-fast positive, galactose
smegmatis                fermentation negative, catalase-negative,, non-motile, alkaline in litmus
                         milk, catalase positive, not hemolytic, H 2S negative

Mycobacterium leprae     Gram positive club-shaped bacilli, acid-fast positive, galactose
                         fermentation negative, catalase-positive, non-motile, alkaline in litmus
                         milk not hemolytic, H2S negative

Corynebacterium          Gram positive club-shaped bacilli, acid-fast negative, galactose
xerosis                  fermentation positive, non-motile, acid from litmus milk, catalase
                         positive, not hemolytic, H2S negative

Aeromonas hydrophila     Gram negative bacilli, acid-fast negative, ferments galactose, catalase
                         positive, motile, acid from litmus milk, not hemolytic, H 2S positive
Dichotomous Key to Identifying Eight Bacteria

    Alcaligenes faecalis       Mycobacterium smegmatis
    Citrobacter freundii       Mycobacterium leprae
    Pseudomonas fluorescens    Corynebacterium xerosis
    Streptococcus pneumoniae   Aeromonas hydrophila
Table of Characteristics for Eight Bacteria
      Organism                                       Characteristics
Alcaligenes faecalis     Gram negative bacillus, acid-fast negative, ferments galactose,
                         catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S
                         negative
Citrobacter freundii     Gram negative bacillus; acid-fast negative, does not ferment galactose,
                         catalase positive, non-motile, acid produced in litmus milk, not
                         hemolytic, H2S negative

Pseudomonas              Gram negative bacillus, acid-fast negative, does not ferment galactose,
fluorescens              catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S
                         negative
Streptococcus            Gram positive oval coccus found as streptococci, acid-fast negative,
pneumoniae               galactose fermentation negative, catalase negative, non-motile, acid in
                         litmus milk, hemolytic, H2S negative

Mycobacterium            Gram positive club-shaped bacilli, acid-fast positive, galactose
smegmatis                fermentation negative, catalase-negative,, non-motile, alkaline in litmus
                         milk, catalase positive, not hemolytic, H 2S negative

Mycobacterium leprae     Gram positive club-shaped bacilli, acid-fast positive, galactose
                         fermentation negative, catalase-positive, non-motile, alkaline in litmus
                         milk not hemolytic, H2S negative

Corynebacterium          Gram positive club-shaped bacilli, acid-fast negative, galactose
xerosis                  fermentation positive, non-motile, acid from litmus milk, catalase
                         positive, not hemolytic, H2S negative

Aeromonas hydrophila     Gram negative bacilli, acid-fast negative, ferments galactose, catalase
                         positive, motile, acid from litmus milk, not hemolytic, H 2S positive
Table of Characteristics for Eight Bacteria
      Organism                                       Characteristics
Alcaligenes faecalis     Gram negative bacillus, acid-fast negative, ferments galactose,
                         catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S
                         negative
Citrobacter freundii     Gram negative bacillus; acid-fast negative, does not ferment galactose,
                         catalase positive, non-motile, acid produced in litmus milk, not
                         hemolytic, H2S negative

Pseudomonas              Gram negative bacillus, acid-fast negative, does not ferment galactose,
fluorescens              catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S
                         negative
Streptococcus            Gram positive oval coccus found as streptococci, acid-fast negative,
pneumoniae               galactose fermentation negative, catalase negative, non-motile, acid in
                         litmus milk, hemolytic, H2S negative

Mycobacterium            Gram positive club-shaped bacilli, acid-fast positive, galactose
smegmatis                fermentation negative, catalase-negative,, non-motile, alkaline in litmus
                         milk, catalase positive, not hemolytic, H 2S negative

Mycobacterium leprae     Gram positive club-shaped bacilli, acid-fast positive, galactose
                         fermentation negative, catalase-positive, non-motile, alkaline in litmus
                         milk not hemolytic, H2S negative

Corynebacterium          Gram positive club-shaped bacilli, acid-fast negative, galactose
xerosis                  fermentation positive, non-motile, acid from litmus milk, catalase
                         positive, not hemolytic, H2S negative

Aeromonas hydrophila     Gram negative bacilli, acid-fast negative, ferments galactose, catalase
                         positive, motile, acid from litmus milk, not hemolytic, H 2S positive
Dichotomous Key to Identifying Eight Bacteria

               Alcaligenes faecalis       Mycobacterium smegmatis
               Citrobacter freundii       Mycobacterium leprae
               Pseudomonas fluorescens    Corynebacterium xerosis
               Streptococcus pneumoniae   Aeromonas hydrophila


                               Gram stain

                negative                      positive
Alcaligenes faecalis                                Mycobacterium smegmatis
Citrobacter freundii                                Mycobacterium leprae
Pseudomonas fluorescens                             Corynebacterium xerosis
Aeromonas hydrophila                                Streptococcus pneumoniae
Table of Characteristics for Eight Bacteria
      Organism                                       Characteristics
Alcaligenes faecalis     Gram negative bacillus, acid-fast negative, ferments galactose,
                         catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S
                         negative
Citrobacter freundii     Gram negative bacillus; acid-fast negative, does not ferment galactose,
                         catalase positive, non-motile, acid produced in litmus milk, not
                         hemolytic, H2S negative

Pseudomonas              Gram negative bacillus, acid-fast negative, does not ferment galactose,
fluorescens              catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S
                         negative
Streptococcus            Gram positive oval coccus found as streptococci, acid-fast negative,
pneumoniae               galactose fermentation negative, catalase negative, non-motile, acid in
                         litmus milk, hemolytic, H2S negative

Mycobacterium            Gram positive club-shaped bacilli, acid-fast positive, galactose
smegmatis                fermentation negative, catalase-negative,, non-motile, alkaline in litmus
                         milk, catalase positive, not hemolytic, H 2S negative

Mycobacterium leprae     Gram positive club-shaped bacilli, acid-fast positive, galactose
                         fermentation negative, catalase-positive, non-motile, alkaline in litmus
                         milk not hemolytic, H2S negative

Corynebacterium          Gram positive club-shaped bacilli, acid-fast negative, galactose
xerosis                  fermentation positive, non-motile, acid from litmus milk, catalase
                         positive, not hemolytic, H2S negative

Aeromonas hydrophila     Gram negative bacilli, acid-fast negative, ferments galactose, catalase
                         positive, motile, acid from litmus milk, not hemolytic, H 2S positive
Dichotomous Key to Identifying Eight Bacteria

                            Alcaligenes faecalis       Mycobacterium smegmatis
                            Citrobacter freundii       Mycobacterium leprae
                            Pseudomonas fluorescens    Corynebacterium xerosis
                            Streptococcus pneumoniae   Aeromonas hydrophila


                                            Gram stain

                             negative                      positive
             Alcaligenes faecalis                                Mycobacterium smegmatis
             Citrobacter freundii                                Mycobacterium leprae
             Pseudomonas fluorescens                             Corynebacterium xerosis
             Aeromonas hydrophila                                Streptococcus pneumoniae

         Galactose fermentation

         positive            negative
Alcaligenes faecalis      Citrobacter freundii
Aeromonas hydrophila      Pseudomonas fluorescens
Table of Characteristics for Eight Bacteria
      Organism                                       Characteristics
Alcaligenes faecalis     Gram negative bacillus, acid-fast negative, ferments galactose,
                         catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S
                         negative
Citrobacter freundii     Gram negative bacillus; acid-fast negative, does not ferment galactose,
                         catalase positive, non-motile, acid produced in litmus milk, not
                         hemolytic, H2S negative

Pseudomonas              Gram negative bacillus, acid-fast negative, does not ferment galactose,
fluorescens              catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S
                         negative
Streptococcus            Gram positive oval coccus found as streptococci, acid-fast negative,
pneumoniae               galactose fermentation negative, catalase negative, non-motile, acid in
                         litmus milk, hemolytic, H2S negative

Mycobacterium            Gram positive club-shaped bacilli, acid-fast positive, galactose
smegmatis                fermentation negative, catalase-negative,, non-motile, alkaline in litmus
                         milk, catalase positive, not hemolytic, H 2S negative

Mycobacterium leprae     Gram positive club-shaped bacilli, acid-fast positive, galactose
                         fermentation negative, catalase-positive, non-motile, alkaline in litmus
                         milk not hemolytic, H2S negative

Corynebacterium          Gram positive club-shaped bacilli, acid-fast negative, galactose
xerosis                  fermentation positive, non-motile, acid from litmus milk, catalase
                         positive, not hemolytic, H2S negative

Aeromonas hydrophila     Gram negative bacilli, acid-fast negative, ferments galactose, catalase
                         positive, motile, acid from litmus milk, not hemolytic, H 2S positive
Dichotomous Key to Identifying Eight Bacteria

                                 Alcaligenes faecalis       Mycobacterium smegmatis
                                 Citrobacter freundii       Mycobacterium leprae
                                 Pseudomonas fluorescens    Corynebacterium xerosis
                                 Streptococcus pneumoniae   Aeromonas hydrophila


                                                 Gram stain

                                  negative                      positive
                  Alcaligenes faecalis                                Mycobacterium smegmatis
                  Citrobacter freundii                                Mycobacterium leprae
                  Pseudomonas fluorescens                             Corynebacterium xerosis
                  Aeromonas hydrophila                                Streptococcus pneumoniae

              Galactose fermentation

              positive            negative
     Alcaligenes faecalis      Citrobacter freundii
     Aeromonas hydrophila      Pseudomonas fluorescens

     H2S production
 positive      negative
Aeromonas      Alcaligenes
hydrophila     faecalis
Table of Characteristics for Eight Bacteria
      Organism                                       Characteristics
Alcaligenes faecalis     Gram negative bacillus, acid-fast negative, ferments galactose,
                         catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S
                         negative
Citrobacter freundii     Gram negative bacillus; acid-fast negative, does not ferment galactose,
                         catalase positive, non-motile, acid produced in litmus milk, not
                         hemolytic, H2S negative

Pseudomonas              Gram negative bacillus, acid-fast negative, does not ferment galactose,
fluorescens              catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S
                         negative
Streptococcus            Gram positive oval coccus found as streptococci, acid-fast negative,
pneumoniae               galactose fermentation negative, catalase negative, non-motile, acid in
                         litmus milk, hemolytic, H2S negative

Mycobacterium            Gram positive club-shaped bacilli, acid-fast positive, galactose
smegmatis                fermentation negative, catalase-negative,, non-motile, alkaline in litmus
                         milk, catalase positive, not hemolytic, H 2S negative

Mycobacterium leprae     Gram positive club-shaped bacilli, acid-fast positive, galactose
                         fermentation negative, catalase-positive, non-motile, alkaline in litmus
                         milk not hemolytic, H2S negative

Corynebacterium          Gram positive club-shaped bacilli, acid-fast negative, galactose
xerosis                  fermentation positive, non-motile, acid from litmus milk, catalase
                         positive, not hemolytic, H2S negative

Aeromonas hydrophila     Gram negative bacilli, acid-fast negative, ferments galactose, catalase
                         positive, motile, acid from litmus milk, not hemolytic, H 2S positive
Dichotomous Key to Identifying Eight Bacteria

                                 Alcaligenes faecalis       Mycobacterium smegmatis
                                 Citrobacter freundii       Mycobacterium leprae
                                 Pseudomonas fluorescens    Corynebacterium xerosis
                                 Streptococcus pneumoniae   Aeromonas hydrophila


                                                 Gram stain

                                  negative                      positive
                  Alcaligenes faecalis                                Mycobacterium smegmatis
                  Citrobacter freundii                                Mycobacterium leprae
                  Pseudomonas fluorescens                             Corynebacterium xerosis
                  Aeromonas hydrophila                                Streptococcus pneumoniae

              Galactose fermentation

              positive            negative
     Alcaligenes faecalis      Citrobacter freundii
     Aeromonas hydrophila      Pseudomonas fluorescens

     H2S production               Litmus milk
 positive      negative       acid           alkaline
Aeromonas      Alcaligenes Citrobacter       Pseudomonas
hydrophila     faecalis    freundii          fluorescens
Table of Characteristics for Eight Bacteria
      Organism                                       Characteristics
Alcaligenes faecalis     Gram negative bacillus, acid-fast negative, ferments galactose,
                         catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S
                         negative
Citrobacter freundii     Gram negative bacillus; acid-fast negative, does not ferment galactose,
                         catalase positive, non-motile, acid produced in litmus milk, not
                         hemolytic, H2S negative

Pseudomonas              Gram negative bacillus, acid-fast negative, does not ferment galactose,
fluorescens              catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S
                         negative
Streptococcus            Gram positive oval coccus found as streptococci, acid-fast negative,
pneumoniae               galactose fermentation negative, catalase negative, non-motile, acid in
                         litmus milk, hemolytic, H2S negative

Mycobacterium            Gram positive club-shaped bacilli, acid-fast positive, galactose
smegmatis                fermentation negative, catalase-negative,, non-motile, alkaline in litmus
                         milk, catalase positive, not hemolytic, H 2S negative

Mycobacterium leprae     Gram positive club-shaped bacilli, acid-fast positive, galactose
                         fermentation negative, catalase-positive, non-motile, alkaline in litmus
                         milk not hemolytic, H2S negative

Corynebacterium          Gram positive club-shaped bacilli, acid-fast negative, galactose
xerosis                  fermentation positive, non-motile, acid from litmus milk, catalase
                         positive, not hemolytic, H2S negative

Aeromonas hydrophila     Gram negative bacilli, acid-fast negative, ferments galactose, catalase
                         positive, motile, acid from litmus milk, not hemolytic, H 2S positive
Dichotomous Key to Identifying Eight Bacteria

                                 Alcaligenes faecalis       Mycobacterium smegmatis
                                 Citrobacter freundii       Mycobacterium leprae
                                 Pseudomonas fluorescens    Corynebacterium xerosis
                                 Streptococcus pneumoniae   Aeromonas hydrophila


                                                 Gram stain

                                  negative                      positive
                  Alcaligenes faecalis                                Mycobacterium smegmatis
                  Citrobacter freundii                                Mycobacterium leprae
                  Pseudomonas fluorescens                             Corynebacterium xerosis
                  Aeromonas hydrophila                                Streptococcus pneumoniae

              Galactose fermentation                                  Acid-fast staining

              positive            negative                          acid fast          not acid fast
                                                            Mycobacterium smegmatis      Corynebacterium xerosis
     Alcaligenes faecalis      Citrobacter freundii
                                                            Mycobacterium leprae         Streptococcus pneumoniae
     Aeromonas hydrophila      Pseudomonas fluorescens

     H2S production               Litmus milk
 positive      negative       acid           alkaline
Aeromonas      Alcaligenes Citrobacter       Pseudomonas
hydrophila     faecalis    freundii          fluorescens
Table of Characteristics for Eight Bacteria
      Organism                                       Characteristics
Alcaligenes faecalis     Gram negative bacillus, acid-fast negative, ferments galactose,
                         catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S
                         negative
Citrobacter freundii     Gram negative bacillus; acid-fast negative, does not ferment galactose,
                         catalase positive, non-motile, acid produced in litmus milk, not
                         hemolytic, H2S negative

Pseudomonas              Gram negative bacillus, acid-fast negative, does not ferment galactose,
fluorescens              catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S
                         negative
Streptococcus            Gram positive oval coccus found as streptococci, acid-fast negative,
pneumoniae               galactose fermentation negative, catalase negative, non-motile, acid in
                         litmus milk, hemolytic, H2S negative

Mycobacterium            Gram positive club-shaped bacilli, acid-fast positive, galactose
smegmatis                fermentation negative, catalase-negative,, non-motile, alkaline in litmus
                         milk, catalase positive, not hemolytic, H 2S negative

Mycobacterium leprae     Gram positive club-shaped bacilli, acid-fast positive, galactose
                         fermentation negative, catalase-positive, non-motile, alkaline in litmus
                         milk not hemolytic, H2S negative

Corynebacterium          Gram positive club-shaped bacilli, acid-fast negative, galactose
xerosis                  fermentation positive, non-motile, acid from litmus milk, catalase
                         positive, not hemolytic, H2S negative

Aeromonas hydrophila     Gram negative bacilli, acid-fast negative, ferments galactose, catalase
                         positive, motile, acid from litmus milk, not hemolytic, H 2S positive
Dichotomous Key to Identifying Eight Bacteria

                                 Alcaligenes faecalis       Mycobacterium smegmatis
                                 Citrobacter freundii       Mycobacterium leprae
                                 Pseudomonas fluorescens    Corynebacterium xerosis
                                 Streptococcus pneumoniae   Aeromonas hydrophila


                                                 Gram stain

                                  negative                      positive
                  Alcaligenes faecalis                                  Mycobacterium smegmatis
                  Citrobacter freundii                                  Mycobacterium leprae
                  Pseudomonas fluorescens                               Corynebacterium xerosis
                  Aeromonas hydrophila                                  Streptococcus pneumoniae

              Galactose fermentation                                    Acid-fast staining

              positive            negative                          acid fast            not acid fast
                                                            Mycobacterium smegmatis        Corynebacterium xerosis
     Alcaligenes faecalis      Citrobacter freundii
                                                            Mycobacterium leprae           Streptococcus pneumoniae
     Aeromonas hydrophila      Pseudomonas fluorescens

     H2S production               Litmus milk                  Catalase produced

 positive      negative       acid           alkaline        positive             negative
Aeromonas      Alcaligenes Citrobacter       Pseudomonas     Mycobacterium      Mycobacterium
hydrophila     faecalis    freundii          fluorescens      leprae             smegmatis
Table of Characteristics for Eight Bacteria
      Organism                                       Characteristics
Alcaligenes faecalis     Gram negative bacillus, acid-fast negative, ferments galactose,
                         catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S
                         negative
Citrobacter freundii     Gram negative bacillus; acid-fast negative, does not ferment galactose,
                         catalase positive, non-motile, acid produced in litmus milk, not
                         hemolytic, H2S negative

Pseudomonas              Gram negative bacillus, acid-fast negative, does not ferment galactose,
fluorescens              catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S
                         negative
Streptococcus            Gram positive oval coccus found as streptococci, acid-fast negative,
pneumoniae               galactose fermentation negative, catalase negative, non-motile, acid in
                         litmus milk, hemolytic, H2S negative

Mycobacterium            Gram positive club-shaped bacilli, acid-fast positive, galactose
smegmatis                fermentation negative, catalase-negative,, non-motile, alkaline in litmus
                         milk, catalase positive, not hemolytic, H 2S negative

Mycobacterium leprae     Gram positive club-shaped bacilli, acid-fast positive, galactose
                         fermentation negative, catalase-positive, non-motile, alkaline in litmus
                         milk not hemolytic, H2S negative

Corynebacterium          Gram positive club-shaped bacilli, acid-fast negative, galactose
xerosis                  fermentation positive, non-motile, acid from litmus milk, catalase
                         positive, not hemolytic, H2S negative

Aeromonas hydrophila     Gram negative bacilli, acid-fast negative, ferments galactose, catalase
                         positive, motile, acid from litmus milk, not hemolytic, H 2S positive
Dichotomous Key to Identifying Eight Bacteria

                                 Alcaligenes faecalis       Mycobacterium smegmatis
                                 Citrobacter freundii       Mycobacterium leprae
                                 Pseudomonas fluorescens    Corynebacterium xerosis
                                 Streptococcus pneumoniae   Aeromonas hydrophila


                                                 Gram stain

                                  negative                      positive
                  Alcaligenes faecalis                                  Mycobacterium smegmatis
                  Citrobacter freundii                                  Mycobacterium leprae
                  Pseudomonas fluorescens                               Corynebacterium xerosis
                  Aeromonas hydrophila                                  Streptococcus pneumoniae

              Galactose fermentation                                    Acid-fast staining

              positive            negative                          acid fast            not acid fast
                                                            Mycobacterium smegmatis        Corynebacterium xerosis
     Alcaligenes faecalis      Citrobacter freundii
                                                            Mycobacterium leprae           Streptococcus pneumoniae
     Aeromonas hydrophila      Pseudomonas fluorescens

     H2S production               Litmus milk                  Catalase produced                    Hemolysis

 positive      negative       acid           alkaline        positive             negative         positive     negative
Aeromonas      Alcaligenes Citrobacter       Pseudomonas     Mycobacterium      Mycobacterium Streptococcus   Corynebacterium
hydrophila                 freundii          fluorescens
               faecalis                                       leprae             smegmatis     pneumoniae         xerosis

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Dichotomous key

  • 2. • Prokaryote • microscopic, unicellular organisms • lack nuclei and membrane-bound organelles
  • 3. Bacterial Structures • Flagella • Pili • Capsule • Plasma Membrane • Cytoplasm • Cell Wall • Lipopolysaccharides • Teichoic Acids • Inclusions • Spores Chapter 4
  • 4.
  • 5. How to Make a Dichotomous Key From a Table of Bacterial Characteristics (for use in running the minimum number of tests in identifying an unknown bacterium)
  • 6. Table of Characteristics for Eight Bacteria Organism Characteristics Alcaligenes faecalis Gram negative bacillus, acid-fast negative, ferments galactose, catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S negative Citrobacter freundii Gram negative bacillus; acid-fast negative, does not ferment galactose, catalase positive, non-motile, acid produced in litmus milk, not hemolytic, H2S negative Pseudomonas Gram negative bacillus, acid-fast negative, does not ferment galactose, fluorescens catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S negative Streptococcus Gram positive oval coccus found as streptococci, acid-fast negative, pneumoniae galactose fermentation negative, catalase negative, non-motile, acid in litmus milk, hemolytic, H2S negative Mycobacterium Gram positive club-shaped bacilli, acid-fast positive, galactose smegmatis fermentation negative, catalase-negative,, non-motile, alkaline in litmus milk, catalase positive, not hemolytic, H 2S negative Mycobacterium leprae Gram positive club-shaped bacilli, acid-fast positive, galactose fermentation negative, catalase-positive, non-motile, alkaline in litmus milk not hemolytic, H2S negative Corynebacterium Gram positive club-shaped bacilli, acid-fast negative, galactose xerosis fermentation positive, non-motile, acid from litmus milk, catalase positive, not hemolytic, H2S negative Aeromonas hydrophila Gram negative bacilli, acid-fast negative, ferments galactose, catalase positive, motile, acid from litmus milk, not hemolytic, H 2S positive
  • 7. Dichotomous Key to Identifying Eight Bacteria Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Streptococcus pneumoniae Aeromonas hydrophila
  • 8. Table of Characteristics for Eight Bacteria Organism Characteristics Alcaligenes faecalis Gram negative bacillus, acid-fast negative, ferments galactose, catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S negative Citrobacter freundii Gram negative bacillus; acid-fast negative, does not ferment galactose, catalase positive, non-motile, acid produced in litmus milk, not hemolytic, H2S negative Pseudomonas Gram negative bacillus, acid-fast negative, does not ferment galactose, fluorescens catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S negative Streptococcus Gram positive oval coccus found as streptococci, acid-fast negative, pneumoniae galactose fermentation negative, catalase negative, non-motile, acid in litmus milk, hemolytic, H2S negative Mycobacterium Gram positive club-shaped bacilli, acid-fast positive, galactose smegmatis fermentation negative, catalase-negative,, non-motile, alkaline in litmus milk, catalase positive, not hemolytic, H 2S negative Mycobacterium leprae Gram positive club-shaped bacilli, acid-fast positive, galactose fermentation negative, catalase-positive, non-motile, alkaline in litmus milk not hemolytic, H2S negative Corynebacterium Gram positive club-shaped bacilli, acid-fast negative, galactose xerosis fermentation positive, non-motile, acid from litmus milk, catalase positive, not hemolytic, H2S negative Aeromonas hydrophila Gram negative bacilli, acid-fast negative, ferments galactose, catalase positive, motile, acid from litmus milk, not hemolytic, H 2S positive
  • 9. Table of Characteristics for Eight Bacteria Organism Characteristics Alcaligenes faecalis Gram negative bacillus, acid-fast negative, ferments galactose, catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S negative Citrobacter freundii Gram negative bacillus; acid-fast negative, does not ferment galactose, catalase positive, non-motile, acid produced in litmus milk, not hemolytic, H2S negative Pseudomonas Gram negative bacillus, acid-fast negative, does not ferment galactose, fluorescens catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S negative Streptococcus Gram positive oval coccus found as streptococci, acid-fast negative, pneumoniae galactose fermentation negative, catalase negative, non-motile, acid in litmus milk, hemolytic, H2S negative Mycobacterium Gram positive club-shaped bacilli, acid-fast positive, galactose smegmatis fermentation negative, catalase-negative,, non-motile, alkaline in litmus milk, catalase positive, not hemolytic, H 2S negative Mycobacterium leprae Gram positive club-shaped bacilli, acid-fast positive, galactose fermentation negative, catalase-positive, non-motile, alkaline in litmus milk not hemolytic, H2S negative Corynebacterium Gram positive club-shaped bacilli, acid-fast negative, galactose xerosis fermentation positive, non-motile, acid from litmus milk, catalase positive, not hemolytic, H2S negative Aeromonas hydrophila Gram negative bacilli, acid-fast negative, ferments galactose, catalase positive, motile, acid from litmus milk, not hemolytic, H 2S positive
  • 10. Dichotomous Key to Identifying Eight Bacteria Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Streptococcus pneumoniae Aeromonas hydrophila Gram stain negative positive Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Aeromonas hydrophila Streptococcus pneumoniae
  • 11. Table of Characteristics for Eight Bacteria Organism Characteristics Alcaligenes faecalis Gram negative bacillus, acid-fast negative, ferments galactose, catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S negative Citrobacter freundii Gram negative bacillus; acid-fast negative, does not ferment galactose, catalase positive, non-motile, acid produced in litmus milk, not hemolytic, H2S negative Pseudomonas Gram negative bacillus, acid-fast negative, does not ferment galactose, fluorescens catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S negative Streptococcus Gram positive oval coccus found as streptococci, acid-fast negative, pneumoniae galactose fermentation negative, catalase negative, non-motile, acid in litmus milk, hemolytic, H2S negative Mycobacterium Gram positive club-shaped bacilli, acid-fast positive, galactose smegmatis fermentation negative, catalase-negative,, non-motile, alkaline in litmus milk, catalase positive, not hemolytic, H 2S negative Mycobacterium leprae Gram positive club-shaped bacilli, acid-fast positive, galactose fermentation negative, catalase-positive, non-motile, alkaline in litmus milk not hemolytic, H2S negative Corynebacterium Gram positive club-shaped bacilli, acid-fast negative, galactose xerosis fermentation positive, non-motile, acid from litmus milk, catalase positive, not hemolytic, H2S negative Aeromonas hydrophila Gram negative bacilli, acid-fast negative, ferments galactose, catalase positive, motile, acid from litmus milk, not hemolytic, H 2S positive
  • 12. Dichotomous Key to Identifying Eight Bacteria Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Streptococcus pneumoniae Aeromonas hydrophila Gram stain negative positive Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Aeromonas hydrophila Streptococcus pneumoniae Galactose fermentation positive negative Alcaligenes faecalis Citrobacter freundii Aeromonas hydrophila Pseudomonas fluorescens
  • 13. Table of Characteristics for Eight Bacteria Organism Characteristics Alcaligenes faecalis Gram negative bacillus, acid-fast negative, ferments galactose, catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S negative Citrobacter freundii Gram negative bacillus; acid-fast negative, does not ferment galactose, catalase positive, non-motile, acid produced in litmus milk, not hemolytic, H2S negative Pseudomonas Gram negative bacillus, acid-fast negative, does not ferment galactose, fluorescens catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S negative Streptococcus Gram positive oval coccus found as streptococci, acid-fast negative, pneumoniae galactose fermentation negative, catalase negative, non-motile, acid in litmus milk, hemolytic, H2S negative Mycobacterium Gram positive club-shaped bacilli, acid-fast positive, galactose smegmatis fermentation negative, catalase-negative,, non-motile, alkaline in litmus milk, catalase positive, not hemolytic, H 2S negative Mycobacterium leprae Gram positive club-shaped bacilli, acid-fast positive, galactose fermentation negative, catalase-positive, non-motile, alkaline in litmus milk not hemolytic, H2S negative Corynebacterium Gram positive club-shaped bacilli, acid-fast negative, galactose xerosis fermentation positive, non-motile, acid from litmus milk, catalase positive, not hemolytic, H2S negative Aeromonas hydrophila Gram negative bacilli, acid-fast negative, ferments galactose, catalase positive, motile, acid from litmus milk, not hemolytic, H 2S positive
  • 14. Dichotomous Key to Identifying Eight Bacteria Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Streptococcus pneumoniae Aeromonas hydrophila Gram stain negative positive Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Aeromonas hydrophila Streptococcus pneumoniae Galactose fermentation positive negative Alcaligenes faecalis Citrobacter freundii Aeromonas hydrophila Pseudomonas fluorescens H2S production positive negative Aeromonas Alcaligenes hydrophila faecalis
  • 15. Table of Characteristics for Eight Bacteria Organism Characteristics Alcaligenes faecalis Gram negative bacillus, acid-fast negative, ferments galactose, catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S negative Citrobacter freundii Gram negative bacillus; acid-fast negative, does not ferment galactose, catalase positive, non-motile, acid produced in litmus milk, not hemolytic, H2S negative Pseudomonas Gram negative bacillus, acid-fast negative, does not ferment galactose, fluorescens catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S negative Streptococcus Gram positive oval coccus found as streptococci, acid-fast negative, pneumoniae galactose fermentation negative, catalase negative, non-motile, acid in litmus milk, hemolytic, H2S negative Mycobacterium Gram positive club-shaped bacilli, acid-fast positive, galactose smegmatis fermentation negative, catalase-negative,, non-motile, alkaline in litmus milk, catalase positive, not hemolytic, H 2S negative Mycobacterium leprae Gram positive club-shaped bacilli, acid-fast positive, galactose fermentation negative, catalase-positive, non-motile, alkaline in litmus milk not hemolytic, H2S negative Corynebacterium Gram positive club-shaped bacilli, acid-fast negative, galactose xerosis fermentation positive, non-motile, acid from litmus milk, catalase positive, not hemolytic, H2S negative Aeromonas hydrophila Gram negative bacilli, acid-fast negative, ferments galactose, catalase positive, motile, acid from litmus milk, not hemolytic, H 2S positive
  • 16. Dichotomous Key to Identifying Eight Bacteria Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Streptococcus pneumoniae Aeromonas hydrophila Gram stain negative positive Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Aeromonas hydrophila Streptococcus pneumoniae Galactose fermentation positive negative Alcaligenes faecalis Citrobacter freundii Aeromonas hydrophila Pseudomonas fluorescens H2S production Litmus milk positive negative acid alkaline Aeromonas Alcaligenes Citrobacter Pseudomonas hydrophila faecalis freundii fluorescens
  • 17. Table of Characteristics for Eight Bacteria Organism Characteristics Alcaligenes faecalis Gram negative bacillus, acid-fast negative, ferments galactose, catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S negative Citrobacter freundii Gram negative bacillus; acid-fast negative, does not ferment galactose, catalase positive, non-motile, acid produced in litmus milk, not hemolytic, H2S negative Pseudomonas Gram negative bacillus, acid-fast negative, does not ferment galactose, fluorescens catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S negative Streptococcus Gram positive oval coccus found as streptococci, acid-fast negative, pneumoniae galactose fermentation negative, catalase negative, non-motile, acid in litmus milk, hemolytic, H2S negative Mycobacterium Gram positive club-shaped bacilli, acid-fast positive, galactose smegmatis fermentation negative, catalase-negative,, non-motile, alkaline in litmus milk, catalase positive, not hemolytic, H 2S negative Mycobacterium leprae Gram positive club-shaped bacilli, acid-fast positive, galactose fermentation negative, catalase-positive, non-motile, alkaline in litmus milk not hemolytic, H2S negative Corynebacterium Gram positive club-shaped bacilli, acid-fast negative, galactose xerosis fermentation positive, non-motile, acid from litmus milk, catalase positive, not hemolytic, H2S negative Aeromonas hydrophila Gram negative bacilli, acid-fast negative, ferments galactose, catalase positive, motile, acid from litmus milk, not hemolytic, H 2S positive
  • 18. Dichotomous Key to Identifying Eight Bacteria Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Streptococcus pneumoniae Aeromonas hydrophila Gram stain negative positive Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Aeromonas hydrophila Streptococcus pneumoniae Galactose fermentation Acid-fast staining positive negative acid fast not acid fast Mycobacterium smegmatis Corynebacterium xerosis Alcaligenes faecalis Citrobacter freundii Mycobacterium leprae Streptococcus pneumoniae Aeromonas hydrophila Pseudomonas fluorescens H2S production Litmus milk positive negative acid alkaline Aeromonas Alcaligenes Citrobacter Pseudomonas hydrophila faecalis freundii fluorescens
  • 19. Table of Characteristics for Eight Bacteria Organism Characteristics Alcaligenes faecalis Gram negative bacillus, acid-fast negative, ferments galactose, catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S negative Citrobacter freundii Gram negative bacillus; acid-fast negative, does not ferment galactose, catalase positive, non-motile, acid produced in litmus milk, not hemolytic, H2S negative Pseudomonas Gram negative bacillus, acid-fast negative, does not ferment galactose, fluorescens catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S negative Streptococcus Gram positive oval coccus found as streptococci, acid-fast negative, pneumoniae galactose fermentation negative, catalase negative, non-motile, acid in litmus milk, hemolytic, H2S negative Mycobacterium Gram positive club-shaped bacilli, acid-fast positive, galactose smegmatis fermentation negative, catalase-negative,, non-motile, alkaline in litmus milk, catalase positive, not hemolytic, H 2S negative Mycobacterium leprae Gram positive club-shaped bacilli, acid-fast positive, galactose fermentation negative, catalase-positive, non-motile, alkaline in litmus milk not hemolytic, H2S negative Corynebacterium Gram positive club-shaped bacilli, acid-fast negative, galactose xerosis fermentation positive, non-motile, acid from litmus milk, catalase positive, not hemolytic, H2S negative Aeromonas hydrophila Gram negative bacilli, acid-fast negative, ferments galactose, catalase positive, motile, acid from litmus milk, not hemolytic, H 2S positive
  • 20. Dichotomous Key to Identifying Eight Bacteria Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Streptococcus pneumoniae Aeromonas hydrophila Gram stain negative positive Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Aeromonas hydrophila Streptococcus pneumoniae Galactose fermentation Acid-fast staining positive negative acid fast not acid fast Mycobacterium smegmatis Corynebacterium xerosis Alcaligenes faecalis Citrobacter freundii Mycobacterium leprae Streptococcus pneumoniae Aeromonas hydrophila Pseudomonas fluorescens H2S production Litmus milk Catalase produced positive negative acid alkaline positive negative Aeromonas Alcaligenes Citrobacter Pseudomonas Mycobacterium Mycobacterium hydrophila faecalis freundii fluorescens leprae smegmatis
  • 21. Table of Characteristics for Eight Bacteria Organism Characteristics Alcaligenes faecalis Gram negative bacillus, acid-fast negative, ferments galactose, catalase positive, motile, alkaline with litmus milk, not hemolytic, H 2S negative Citrobacter freundii Gram negative bacillus; acid-fast negative, does not ferment galactose, catalase positive, non-motile, acid produced in litmus milk, not hemolytic, H2S negative Pseudomonas Gram negative bacillus, acid-fast negative, does not ferment galactose, fluorescens catalase positive, non-motile, alkaline in litmus milk, hemolytic, H 2S negative Streptococcus Gram positive oval coccus found as streptococci, acid-fast negative, pneumoniae galactose fermentation negative, catalase negative, non-motile, acid in litmus milk, hemolytic, H2S negative Mycobacterium Gram positive club-shaped bacilli, acid-fast positive, galactose smegmatis fermentation negative, catalase-negative,, non-motile, alkaline in litmus milk, catalase positive, not hemolytic, H 2S negative Mycobacterium leprae Gram positive club-shaped bacilli, acid-fast positive, galactose fermentation negative, catalase-positive, non-motile, alkaline in litmus milk not hemolytic, H2S negative Corynebacterium Gram positive club-shaped bacilli, acid-fast negative, galactose xerosis fermentation positive, non-motile, acid from litmus milk, catalase positive, not hemolytic, H2S negative Aeromonas hydrophila Gram negative bacilli, acid-fast negative, ferments galactose, catalase positive, motile, acid from litmus milk, not hemolytic, H 2S positive
  • 22. Dichotomous Key to Identifying Eight Bacteria Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Streptococcus pneumoniae Aeromonas hydrophila Gram stain negative positive Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Aeromonas hydrophila Streptococcus pneumoniae Galactose fermentation Acid-fast staining positive negative acid fast not acid fast Mycobacterium smegmatis Corynebacterium xerosis Alcaligenes faecalis Citrobacter freundii Mycobacterium leprae Streptococcus pneumoniae Aeromonas hydrophila Pseudomonas fluorescens H2S production Litmus milk Catalase produced Hemolysis positive negative acid alkaline positive negative positive negative Aeromonas Alcaligenes Citrobacter Pseudomonas Mycobacterium Mycobacterium Streptococcus Corynebacterium hydrophila freundii fluorescens faecalis leprae smegmatis pneumoniae xerosis